Abstract:

Platelet concentrates derived from autologous blood are used to stimulate tissue regeneration through the controlled release of growth factors and biologically active proteins. These autologous biomaterials provide a natural fibrin matrix containing platelets, leukocytes, and growth factors that are gradually released and support tissue repair processes. In recent years, they have been applied in the biological treatment of reversible pulpitis, where the main goal is to preserve the vitality of the dental pulp and to stimulate its regenerative potential. In this process, a key role is played by dental pulp stem cells (DPSCs), which possess the ability to proliferate, migrate, and differentiate into odontoblast-like cells under the influence of bioactive molecules released from PRF. The present review aims to outline the trends in the development of PRF-based autologous biomaterials and the experimental methods used for their investigation under laboratory conditions with cell cultures derived from dental pulp stem cells of permanent teeth (DPSCs). The establishment and identification of such stem cell cultures provide an opportunity to study their migration and behaviour in media containing autologous biomaterials, with the purpose of demonstrating their biostimulating activity and potential role in pulp tissue regeneration.

Keywords: Dental pulp; Stem cells, DPSCs, PRF, Growth factors, Regenerative endodontics, Platelet concentrates, Autologous biomaterials