Abstract:

Technologies for tissue engineering are applied for the regeneration of dental pulp. They are based on the use of suitable and accessible cellular sources such as mesenchymal stem cells of dental origin. The creation of cell cultures from stem cells from the dental pulp of permanent teeth and their characterization as such is an important part of carrying out studies on different biomaterials derived from autologous blood.
The aim of the present article is the isolation and cultivation of dental pulp stem cells from permanent teeth and verification of the identity of the obtained cell cultures by immunofluorescent staining. Materials and Methods. Dental pulp was removed from freshly extracted healthy human teeth. By a laboratory protocol, cell cultures of dental pulp stem cells were obtained. They were examined by immunofluorescent staining and analysis of the expression of specific mesenchymal, pluripotent, odontogenic and epithelial markers.
Results. The cells observed under an inverted phase-contrast microscope and the data obtained from the staining demonstrate the mesenchymal origin of the cell cultures.
Conclusion. The results of the present study confirm that, according to the described methodology, the created cell cultures from stem cells are mesenchymal stem cells with odontogenic potential. The application of this methodology in scientific research provides a basis for studying the qualities of autologous blood-derived biomaterials for regenerative restoration of dental pulp affected by an inflammatory process.

Keywords: dental pulp stem cells, mesenchymal stem cells, immunofluorescence, regenerative endodontics, dental pulp, odontogenic differentiation