Abstract:

Ozone therapy is a method used for treatment and prevention across various areas of general and
dental medicine. Ozone therapy has a well-substantiated biological mechanism (the Keap1/Nrf2–
GSH axis) which, at low, controlled doses, triggers an adaptive antioxidant response and yields
antimicrobial, anti-inflammatory, and biostimulatory effects. In cariesology, ozone effectively
reduces the microbial load in demineralized dentin (including resistant strains such as E. faecalis
and C. albicans), supports remineralization, and increases enamel microhardness.
In endodontics, ozone complements standard root canal disinfection, penetrates hard-to-reach
areas, and can support regenerative processes; under controlled parameters it stimulates the
proliferation and osteo-/chondrogenic differentiation of hDPSCs.
In summary, when appropriately dosed and indicated, ozone therapy is a promising, biologically
active adjuvant with strong potential in regenerative dental medicine and infection control—
provided that parameters are strictly controlled and the approach is integrated into evidencebased clinical protocols.endodontics
Keywords: ozone therapy, antimicrobial activity, dental pulp stem cells, endodontic treatment,
caries management, antimicrobial, mineralization